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KMID : 0613820110210091214
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Journal of Life Science
2011 Volume.21 No. 9 p.1214 ~ p.1218
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Antioxidant Activity of Rubus crataegifolius Bge. Fruit Extracts
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Moon Kyoung-Mi
Kim Ji-Eun
Kim Hae-Young
Lee Jae-Seol
Son Gi-Ae
Nam Soo-Wan
Kim Byung-Woo
Lee Jong-Hwan
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Abstract
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We investigated the fruits of Rubus crataegifolius Bge, a plant which has been traditionally used in Korea in phytotherapy, to describe antioxidant materials from plant sources. R. crataegifolius fruits were extracted with methanol and further fractionated into n-hexane, diethyl ether, and ethyl acetate. The antioxidant activity of each fraction and the residue was assessed using a 1,1-diphenyl-2-picrylhydrazyl (DPPH), H₂O₂ radical scavenging method, and their cytotoxicity on human primary kerationcyte (HK) was determined by an MTS assay. The R. crataegifolius fruit methanol extract showed strong antioxidant activity (75.04%, 50%) compared with vitamin C (79.9%, 54.1%) by the DPPH, and H₂O₂ method, respectively. The measured activity from the subsequent extracts of the methanol extract were 20.3% for n-hexane fraction (HF), 68.8% for diethyl ether fraction (DF), 67.1% for ethyl acetate fraction (EF), and 67.1% for the residue fraction (RE) by DPPH and 2.2% for HF, 1.6% for DF, 10% for EF, and 50% for the RE by H₂O₂ assay. An oxidative stress model of HK was established under a suitable concentration (1 mM). The cell viability of the RE treated group increased and the percentage of apoptotic cells decreased at concentrations of 0.005-0.02% RE compared with the H₂O₂ treated group. Fruit extracts of the medicinal plant R. crataegifolius showed potent antioxidant activity and the ability to relieve cell damage from H₂O₂ induced injury to HK.
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KEYWORD
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Antioxidant activity, R. crataegifolius, fractionation, keratinocyte, skin disease
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